Yap W, Schaffer R
Clin Chem. 1977 Jun;23(6):986-9.
We describe a procedure for measuring the binding of small molecules to proteins by means of a dynamic ultrafiltration system. A continuous binding curve is obtained by continuously monitoring the absorbance of ultrafiltrate as a function of ultrafiltrate volume, and corrections are made for the retention of the small molecule by the membrane and the leakage of the protein through the membrane. The method is applied to the binding of bromcresol green to human albumin. Some major sources of uncertainties in the method are discussed.
我们描述了一种通过动态超滤系统测量小分子与蛋白质结合的方法。通过连续监测超滤液的吸光度随超滤液体积的变化来获得连续的结合曲线，并对小分子在膜上的保留以及蛋白质透过膜的泄漏进行校正。该方法应用于溴甲酚绿与人血清白蛋白的结合研究。文中讨论了该方法中一些主要的不确定来源。
