Plesher C J
Clin Chem. 1978 Nov;24(11):2036-9.
Albumin reacts with bromcresol green reagent almost immediately, other serum proteins more slowly. This phenomenon can be exploited to improve the specificity of this routine method for determining serum albumin. I describe a 30-s reaction as adapted to continuous-flow analyzers, with no interference from other serum proteins. The method shows linearity to 72 g/liter. Results of this method correlate very well with those by the Laurell "rocket" and manual (30-s) bromcresol green methods. This procedure gives a considerable improvement in specificity for continuous-flow analyzers as compared with current bromcresol green methods.
白蛋白与溴甲酚绿试剂几乎立即发生反应，其他血清蛋白反应则较慢。可利用这一现象提高这种测定血清白蛋白常规方法的特异性。我描述了一种适用于连续流动分析仪的30秒反应方法，不受其他血清蛋白的干扰。该方法在72克/升范围内呈线性。此方法的结果与劳雷尔“火箭”法和手工（30秒）溴甲酚绿法的结果相关性非常好。与目前的溴甲酚绿法相比，该程序显著提高了连续流动分析仪的特异性。
