Cheshire I M, Blight A, Proops D W
Skin Culture Laboratory, Birmingham General Hospital, UK.
Clin Otolaryngol Allied Sci. 1995 Oct;20(5):453-60. doi: 10.1111/j.1365-2273.1995.tb00081.x.
The ability of cholesteatoma to grow rapidly within the ear is well recognized. This study aimed to quantify the in vitro growth of cholesteatoma derived cells. Following removal of cholesteatoma explant cultures were established. Cellular outgrowths were subcultured and a colony forming assay performed. Cells were repeatedly passaged every 14 days until senescence was observed. In comparison with cells derived from normal scalp skin, cholesteatoma derived cells demonstrated a lower colony forming efficiency in both primary and secondary subcultures, achieved fewer passages and cell generations in serial culture, and achieved a lower total population expansion. No evidence was found to suggest an intrinsic loss of growth control. It is proposed that the majority of cholesteatoma is composed of cells with a limited capacity for growth. Explant studies suggested that these may be the progeny of more highly proliferative cells situated at the neck of the cholesteatoma sac.
胆脂瘤在耳内迅速生长的能力已得到充分认识。本研究旨在量化胆脂瘤来源细胞的体外生长情况。切除胆脂瘤后建立外植体培养。将细胞生长物进行传代培养并进行集落形成试验。每14天对细胞进行反复传代，直至观察到衰老。与正常头皮皮肤来源的细胞相比，胆脂瘤来源的细胞在原代和二代传代培养中均表现出较低的集落形成效率，在连续培养中传代次数和细胞代数较少，且总群体扩增较低。未发现有证据表明存在生长控制的内在丧失。有人提出，大多数胆脂瘤由生长能力有限的细胞组成。外植体研究表明，这些细胞可能是位于胆脂瘤囊颈部增殖能力更强的细胞的后代。
