Pariseau J F, Leblond F A, Harel F, Lepage Y, Hallé J P
Centre de Recherche Guy Bernier, Hôpital Maisonneuve-Rosemont, Montréal, Québec, Canada.
J Biomed Mater Res. 1995 Nov;29(11):1331-5. doi: 10.1002/jbm.820291104.
The study of microcapsule biocompatibility is hindered by their uneven distribution and low recovery when implanted into the peritoneum. We evaluated the use of the rat epididymal fat pad as a microcapsule implantation site for biocompatibility studies. The recovery rate of microcapsules containing 85Sr-labeled microspheres was 99.6 +/- 0.75%. Microcapsules made from the same batch of nonpurified alginate, were injected into both fat pads of male Lewis rats (n = 18) and retrieved 14 days later. A semiquantitative fibrosis score scaled from 0 to 3.0 showed that the pericapsular reaction was uniform throughout a fat pad, and that the results of the two fat pads were equivalent because the null hypothesis of inequivalence was rejected (P < .001). Thus, this method can be used to compare the biocompatibility of microcapsule of differing compositions.
微胶囊植入腹膜后，其分布不均和回收率低阻碍了微胶囊生物相容性的研究。我们评估了将大鼠附睾脂肪垫作为微胶囊植入部位用于生物相容性研究的可行性。含有85Sr标记微球的微胶囊回收率为99.6±0.75%。将同一批次未纯化藻酸盐制成的微胶囊注射到雄性Lewis大鼠（n = 18）的双侧脂肪垫中，并在14天后取出。半定量纤维化评分范围为0至3.0，结果显示脂肪垫内整个囊周反应均匀，且由于不等效性的零假设被拒绝（P <.001），两个脂肪垫的结果相当。因此，该方法可用于比较不同成分微胶囊的生物相容性。
