Schugens C, Grandfils C, Jerome R, Teyssie P, Delree P, Martin D, Malgrange B, Moonen G
Center for Education and Research on Macromolecules (CERM), University of Liège, Belgium.
J Biomed Mater Res. 1995 Nov;29(11):1349-62. doi: 10.1002/jbm.820291106.
This article reports the production of a surgical implant meeting several specific requirements such as biocompatibility, biodegradability, macroporosity, and flexibility. Porosity was controlled by an original method consisting of the aggregation of calibrated poly-D,L-lactide microparticles. The size of the interstices between the aggregated microspheres was in a direct relationship to the microsphere diameter. A first approach was based on coating the microspheres with poly(vinyl alcohol) followed by chemically crosslinking the coating layers that were in mutual contact. This method was disregarded because of the acute cytotoxicity of glutaraldehyde used as the crosslinking agent, the absence of macroporosity, and the complete lack of flexibility. A physical technique of aggregation was then tested, which relied on the plasticization of poly-D,L-lactide microspheres with triethylcitrate to the point where microspheres strongly adhered to each other when they were in contact. This method has proved to be straightforward and definitely superior to the chemical approach, particularly with respect to cytotoxicity, control of macroporosity, and flexibility. A polymer support was thus successfully which was biodegradable, macroporous( interconnected pores of 10-100 microns in diameter), and flexible. This potential medical device is presently being used for neuronal transplantation in the central nervous system.
本文报道了一种满足生物相容性、生物可降解性、大孔性和柔韧性等多项特定要求的外科植入物的生产情况。孔隙率通过一种由校准的聚-D,L-丙交酯微粒聚集组成的原始方法进行控制。聚集微球之间的间隙大小与微球直径直接相关。第一种方法是基于用聚乙烯醇涂覆微球，然后对相互接触的涂层进行化学交联。由于用作交联剂的戊二醛具有急性细胞毒性、缺乏大孔性以及完全缺乏柔韧性，该方法被摒弃。随后测试了一种物理聚集技术，该技术依靠用柠檬酸三乙酯使聚-D,L-丙交酯微球增塑，直至微球在接触时彼此强烈粘附。事实证明，这种方法简单直接，并且在细胞毒性、大孔性控制和柔韧性方面绝对优于化学方法。因此成功制备了一种聚合物载体，它具有生物可降解性、大孔性（直径为10 - 100微米的相互连通的孔）和柔韧性。这种潜在的医疗设备目前正用于中枢神经系统的神经元移植。
