Scragg M A, Turton L R, Newman H N, Williams D M
Department of Oral Pathology, London Hospital Medical College, UK.
J Clin Periodontol. 1995 Aug;22(8):585-90. doi: 10.1111/j.1600-051x.1995.tb00809.x.
The effects of Porphyromonas gingivalis culture supernatant from strain W50, its avirulent variant (W50/BE1) and sterile BM culture medium on the morphological changes in polymorphonuclear leucocytes (PMN) from 10 patients with periodontitis (3 juvenile periodontitis, 7 rapidly progressive periodontitis) were compared with those from 10 healthy controls. Large non-polar cells ( > 160 microns2) were increased in patients to 182.8% (p = 0.0076) and 245.5% (p = 0.0002) of control values with W50 and W50/BE1 supernatant respectively. In contrast, numbers of small non-polar cells ( < 80 microns2) and polarised cells were decreased in the patient group. Patient/control ratios for small non-polar cells were 30.3% (p = 0.0007) and 33.6% (p = 0.0005) with W50 and W50/BE1, respectively, and 38.2% (p = 0.0147) for polarised cells exposed to W50 supernatant. The data indicate that PMN from patients with periodontitis differ significantly from those of healthy controls in the shape changes which occur after exposure to P. gingivalis culture supernatant.
比较了牙龈卟啉单胞菌W50菌株及其无毒变体（W50/BE1）的培养上清液以及无菌基础培养基对10例牙周炎患者（3例青少年牙周炎，7例快速进展性牙周炎）和10例健康对照者多形核白细胞（PMN）形态变化的影响。在分别使用W50和W50/BE1上清液处理后，患者组中大型非极性细胞（>160平方微米）增加至对照值的182.8%（p = 0.0076）和245.5%（p = 0.0002）。相反，患者组中小型非极性细胞（<80平方微米）和极化细胞的数量减少。使用W50和W50/BE1处理后，患者组中小型非极性细胞与对照组的比例分别为30.3%（p = 0.0007）和33.6%（p = 0.0005），暴露于W50上清液的极化细胞与对照组的比例为38.2%（p = 0.0147）。数据表明，牙周炎患者的PMN在暴露于牙龈卟啉单胞菌培养上清液后发生的形态变化与健康对照者有显著差异。
