铝佐剂对猪脂多糖全身反应的影响。

Effects of aluminum adjuvant on systemic reactions of lipopolysaccharides in swine.

作者信息

Norimatsu M, Ogikubo Y, Aoki A, Takahashi T, Watanabe G, Taya K, Sasamoto S, Tsuchiya M, Tamura Y

机构信息

National Veterinary Assay Laboratory, Tokyo, Japan.

出版信息

Vaccine. 1995 Oct;13(14):1325-9. doi: 10.1016/0264-410x(95)00023-t.

DOI:10.1016/0264-410x(95)00023-t

PMID:8585288

Abstract

In vivo effects of aluminum adjuvant on systemic reaction of bacterial lipopolysaccharide (LPS) in piglets were investigated. Intramuscular injection of 0.1 mg kg-1 of LPS added to aluminum hydroxide gel (LPS(+)AL) mitigated the leukopenia, trembling and serum levels of TNF-alpha and cortisol compared with the injection of LPS suspended in LPS-free saline (LPS(+)SALINE). The serum endotoxin levels were reduced remarkably but relatively long-lasting in the LPS(+)AL. The lethality in mice injected with LPS added to aluminum hydroxide gel was significantly reduced. Likewise, the Limulus activity of a test LPS was reduced by the addition of aluminum hydroxide gel or aluminum chloride.

摘要

研究了铝佐剂对仔猪细菌脂多糖（LPS）全身反应的体内效应。与注射悬浮于无LPS盐水中的LPS（LPS(+)SALINE）相比，肌肉注射添加到氢氧化铝凝胶中的0.1 mg kg-1 LPS（LPS(+)AL）减轻了白细胞减少、颤抖以及TNF-α和皮质醇的血清水平。LPS(+)AL中的血清内毒素水平显著降低但持续时间相对较长。注射添加到氢氧化铝凝胶中的LPS的小鼠致死率显著降低。同样，添加氢氧化铝凝胶或氯化铝可降低测试LPS的鲎试剂活性。