Sloan L, Schneider S, Rosenblatt J
Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota 55905, USA.
J Clin Microbiol. 1995 Dec;33(12):3124-8. doi: 10.1128/jcm.33.12.3124-3128.1995.
We evaluated a commercially available enzyme-linked immunoassay (ELISA) from LMD Laboratories, Inc., Carlsbad, Calif., for the detection of antibodies in serum to the cysticercus of Taenia solium. The ELISA was performed on 308 serum samples; 198 from a pool of healthy individuals, 42 from patients who had antibodies against a variety of parasites other than T. solium, and 68 from patients suspected of having cysticercosis. All of these 68 specimens were tested both by the ELISA and by an immunoblot method (enzyme-linked immunoelectrotransfer blot assay [EITB]) developed at the Parasitic Serology Laboratory of the Centers for Disease Control and Prevention. Twenty-seven of the 68 serum samples from patients suspected of having cysticercosis were positive by both EITB and ELISA, while 31 were negative by both assays. ELISA results for three and two samples were considered false positive and false negative, respectively, when compared with the results of EITB. Results for an additional five samples were considered equivocal but were technically positive because their optical density readings were slightly above the cutoff value. Three of the 198 serum samples from the bank of serum samples from healthy individuals were also false positive by ELISA (the EITB result for the samples was negative). Six other serum samples from healthy individuals which had equivocal results and the five serum samples from patients with equivocal results were EITB negative. Serum samples containing antibodies against Echinococcus spp. frequently cross-reacted with the cysticercus ELISA antigen (13 of 16 specimens), but serum samples with antibodies against other parasites did not (2 of 26 specimens); all of these serum samples were EITB negative. The commercially available ELISA that we describe is a simple and rapid test. Considering all 308 specimens, the ELISA had a specificity of 93% (when samples with equivocal results were considered negative) or 89% (when samples with equivocal results were considered positive); the sensitivity was 93%.
我们评估了加利福尼亚州卡尔斯巴德市LMD实验室公司生产的一种商用酶联免疫吸附测定法（ELISA），用于检测血清中针对猪带绦虫囊尾蚴的抗体。对308份血清样本进行了ELISA检测；其中198份来自一组健康个体，42份来自患有除猪带绦虫外多种寄生虫抗体的患者，68份来自疑似患有囊尾蚴病的患者。这68份标本均通过ELISA和疾病控制与预防中心寄生虫血清学实验室开发的免疫印迹法（酶联免疫电转移印迹测定法[EITB]）进行检测。68份疑似囊尾蚴病患者的血清样本中，27份经EITB和ELISA检测均为阳性，31份两种检测均为阴性。与EITB结果相比，ELISA检测结果中分别有3份和2份样本被认为是假阳性和假阴性。另外5份样本的结果被认为不明确，但由于其光密度读数略高于临界值，在技术上为阳性。198份健康个体血清样本库中的3份样本经ELISA检测也为假阳性（这些样本的EITB结果为阴性）。另外6份结果不明确的健康个体血清样本和5份结果不明确的患者血清样本EITB检测为阴性。含有抗棘球绦虫属抗体的血清样本经常与囊尾蚴ELISA抗原发生交叉反应（16份标本中的13份），但含有抗其他寄生虫抗体的血清样本则不会（26份标本中的2份）；所有这些血清样本EITB检测均为阴性。我们所描述的这种商用ELISA是一种简单快速的检测方法。考虑所有308份标本，ELISA的特异性为93%（将结果不明确的样本视为阴性时）或89%（将结果不明确的样本视为阳性时）；灵敏度为93%。
