干血斑中巨细胞病毒（CMV）DNA定量评估：CMV先天性感染的回顾性研究
Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection.
作者信息
Vauloup-Fellous Christelle, Ducroux Aurélie, Couloigner Vincent, Marlin Sandrine, Picone Olivier, Galimand Julie, Loundon Natalie, Denoyelle Françoise, Grangeot-Keros Liliane, Leruez-Ville Marianne
机构信息
Service de Microbiologie Immunologie Biologique, Assistance Publique-Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, Clamart, France.
出版信息
J Clin Microbiol. 2007 Nov;45(11):3804-6. doi: 10.1128/JCM.01654-07. Epub 2007 Sep 26.
Abstract
We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.
摘要
我们比较了两种从干血斑中提取DNA的方法，用于通过实时PCR检测和定量巨细胞病毒（CMV）DNA。两种提取方法对于CMV先天性感染的回顾性诊断都是可靠的。在用白蛋白基因PCR扩增结果对病毒载量进行标准化后，CMV DNA的定量分析很有价值。
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本文引用的文献
1
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2
Diagnosis of human congenital cytomegalovirus infection by amplification of viral DNA from dried blood spots on perinatal cards.
J Mol Diagn. 2006 May;8(2):240-5. doi: 10.2353/jmoldx.2006.050075.
3
Multicity Italian study of congenital cytomegalovirus infection.先天性巨细胞病毒感染的多中心意大利研究。
Pediatr Infect Dis J. 2006 Feb;25(2):156-9. doi: 10.1097/01.inf.0000199261.98769.29.
4
Neonatal cytomegalovirus blood load and risk of sequelae in symptomatic and asymptomatic congenitally infected newborns.有症状和无症状先天性感染新生儿的新生儿巨细胞病毒血载量及后遗症风险
Pediatrics. 2006 Jan;117(1):e76-83. doi: 10.1542/peds.2005-0629. Epub 2005 Dec 1.
5
Congenital cytomegalovirus infection: association between virus burden in infancy and hearing loss.先天性巨细胞病毒感染：婴儿期病毒载量与听力损失之间的关联
J Pediatr. 2005 Jun;146(6):817-23. doi: 10.1016/j.jpeds.2005.01.059.
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J Clin Microbiol. 2003 May;41(5):2040-6. doi: 10.1128/JCM.41.5.2040-2046.2003.
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