Yamamoto Y, Kohno S, Koga H, Kakeya H, Tomono K, Kaku M, Yamazaki T, Arisawa M, Hara K
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
J Clin Microbiol. 1995 Dec;33(12):3328-32. doi: 10.1128/jcm.33.12.3328-3332.1995.
We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six patterns among 21 clinical isolates and three patterns among 8 environmental isolates. Pattern I was the most common (18 of 29 isolates) and was found among isolates obtained throughout the entire Nagasaki Prefecture. Patterns I, III, and IV were found among both clinical and environmental isolates. Patterns I and IV had a characteristic distribution, and in particular, pattern IV was isolated exclusively (five of six isolates) from isolates from Nagasaki City. Two environmental isolates from two locations associated strongly with two patients revealed identical random amplified polymorphic DNA patterns (patterns I and IV) for isolates from each patient. Our results suggest that clinical and environmental isolates belong to the same pool of C. neoformans isolates and that these isolates have certain geographic locations, although the number of isolated strains was limited.
我们对日本长崎县南部的新型隐球菌新生变种（血清型A）的临床分离株（肺隐球菌病和隐球菌性脑膜炎）及环境分离株（鸽粪）进行了研究。使用三种引物获得的随机扩增多态性DNA图谱显示，21株临床分离株中有六种图谱，8株环境分离株中有三种图谱。图谱I最为常见（29株分离株中有18株），在整个长崎县获得的分离株中均有发现。图谱I、III和IV在临床和环境分离株中均有发现。图谱I和IV具有特征性分布，特别是图谱IV仅从长崎市的分离株中分离得到（6株分离株中有5株）。来自与两名患者密切相关的两个地点的两株环境分离株，其各自患者的分离株显示出相同的随机扩增多态性DNA图谱（图谱I和IV）。我们的结果表明，临床和环境分离株属于同一新型隐球菌分离株库，并且这些分离株具有一定的地理位置分布，尽管分离菌株的数量有限。
