补充 N-3 多不饱和脂肪酸的饮食对大鼠中性粒细胞介导的回肠通透性和中性粒细胞功能的影响。

Effect of N-3 polyunsaturated fatty acid supplemented diet on neutrophil-mediated ileal permeability and neutrophil function in the rat.

作者信息

Chawla A, Karl P I, Fisher S E

机构信息

Department of Pediatrics, North Shore University Hospital-Cornell University Medical College, Manhasset, New York, USA.

出版信息

J Am Coll Nutr. 1995 Jun;14(3):258-63. doi: 10.1080/07315724.1995.10718505.

DOI:10.1080/07315724.1995.10718505

PMID:8586775

Abstract

RATIONALE AND OBJECTIVE

Fish oil, rich in n-3 polyunsaturated fatty acids, can alter leukotriene production and hence neutrophil function, factors which may be important in the inflammation of Crohn's disease (CD). Therefore we studied the effect of dietary PUFA on neutrophil mediated ileal inflammation and neutrophil function in the rat.

METHODS

Animals were ad libitum-fed pellet diets containing 9.5% fish oil (menhaden oil, rich in n-3 PUFA) with 0.5% safflower oil, 10% safflower oil (rich in n-6 PUFA) or standard chow (6% fat) for 50 days prior to the study. Weight and circulating leukocyte and total neutrophil counts were identical in all three groups. Neutrophil mediated ileal inflammation induced by formyl-methionyl-leucyl-phenylalanine (fMLP) perfusion was evaluated by measuring macromolecular uptake of radiolabelled dextran (MW 70,000) and changes in mucosal neutrophil infiltration.

RESULTS

The fish oil diet group showed no difference in fMLP-induced permeability changes relative to the Chow Control group. However, the Safflower Oil supplemented diet group had a reduced permeability response (p < 0.01). Mirroring the permeability changes, there was diminished mucosal neutrophil infiltration in the Safflower Oil group following ileal perfusion with fMLP (< .005). Chemotaxis and chemiluminescence, two important neutrophil functions, were also significantly suppressed in the Safflower Oil animals (p < 0.05).

CONCLUSIONS

The failure of a n-3 PUFA enriched diet to diminish the ileal inflammatory response to a bacterial peptide and suppress neutrophil function in the rat suggests such therapy would not be expected to be highly successful in CD. However, it requires confirmation in man, especially under the more complicated inflammatory conditions found in CD. On the other hand, the decreased neutrophil mediated responses with a high linoleic acid (n-6 PUFA) diet warrant further investigation.

摘要

原理与目的

富含n-3多不饱和脂肪酸的鱼油可改变白三烯生成，进而影响中性粒细胞功能，这些因素可能在克罗恩病（CD）炎症中起重要作用。因此，我们研究了饮食中多不饱和脂肪酸（PUFA）对大鼠中性粒细胞介导的回肠炎症及中性粒细胞功能的影响。

方法

在研究前50天，动物随意进食含9.5%鱼油（鲱鱼油，富含n-3 PUFA）与0.5%红花油、10%红花油（富含n-6 PUFA）或标准饲料（6%脂肪）的颗粒饲料。三组动物的体重、循环白细胞及中性粒细胞总数相同。通过测量放射性标记葡聚糖（分子量70,000）的大分子摄取及黏膜中性粒细胞浸润变化，评估甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）灌注诱导的中性粒细胞介导的回肠炎症。

结果

与正常对照组相比，鱼油饮食组在fMLP诱导的通透性变化方面无差异。然而，补充红花油的饮食组通透性反应降低（p < 0.01）。与通透性变化一致，回肠灌注fMLP后，红花油组黏膜中性粒细胞浸润减少（< 0.005）。趋化性和化学发光这两项重要的中性粒细胞功能在补充红花油的动物中也受到显著抑制（p < 0.05）。