Furlong E B, Soares L M, Lasca C C, Kohara E Y
Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos, São Paulo, Brazil.
Mycopathologia. 1995 Sep;131(3):185-90. doi: 10.1007/BF01102899.
Wheat from two cultivars with contrasting characteristics were harvested in ten experimental plots located in wheat producing areas of the State of São Paulo, Brazil. The samples (10 of each cultivar) were analyzed by a gas-chromatographic method for deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS), toxins T-2 (T-2) and HT-2, T-2 tetraol, T-2 triol, and by a thin-layer chromatographic method for zearalenone (ZEN), aflatoxins B1, B2, G1, G2, ochratoxin A and sterigmatocystin. No mycotoxins were detected in 13 samples. DON was found in four samples (0.47-0.59 microgram/g), NIV in three samples (0.16-0.40 microgram/g), T-2 in two samples (0.40, 0.80 microgram/g), DAS in one sample (0.60 microgram/g), and ZEN in three samples (0.04-0.21 microgram/g). The wheat samples were also examined for the incidence of fungi. Alternaria, Drechslera, Epicoccum and Cladosporium were the prevailing genera. Among the Fusarium spp., F. semitectum was present in 19 samples and F. moniliforme in 18 samples. No F. graminearum was isolated in the samples.
从巴西圣保罗州小麦产区的10个试验田中收获了两个具有不同特性的小麦品种。对样品（每个品种10个）采用气相色谱法分析脱氧雪腐镰刀菌烯醇（DON）、雪腐镰刀菌烯醇（NIV）、二醋酸镳草镰刀菌烯醇（DAS）、毒素T-2（T-2）和HT-2、T-2四醇、T-2三醇，并采用薄层色谱法分析玉米赤霉烯酮（ZEN）、黄曲霉毒素B1、B2、G1、G2、赭曲霉毒素A和杂色曲霉素。13个样品中未检测到霉菌毒素。在4个样品中发现了DON（0.47 - 0.59微克/克），3个样品中发现了NIV（0.16 - 0.40微克/克），2个样品中发现了T-2（0.40、0.80微克/克），1个样品中发现了DAS（0.60微克/克），3个样品中发现了ZEN（0.04 - 0.21微克/克）。还对小麦样品进行了真菌发生率检测。链格孢属、德氏霉属、附球菌属和枝孢属是优势属。在镰刀菌属中，19个样品中存在半裸镰刀菌，18个样品中存在串珠镰刀菌。样品中未分离出禾谷镰刀菌。
