Scott I, Stear M J, McKellar Q A
Glasgow University Veterinary School, Bearsden.
Res Vet Sci. 1995 Nov;59(3):234-7. doi: 10.1016/0034-5288(95)90009-8.
Four methods for estimating plasma pepsinogen concentrations were compared to determine the most suitable for routine use. Three of the methods were colorimetric assays which used glycine-buffered albumin, albumin, or haemoglobin as their substrates. The fourth method was a radial diffusion assay which used casein in agarose as its substrate. All four methods gave reproducible results when replicates were assayed on the same day. However, the first method had the least day-to-day variation and was the only method which gave a linear relationship with different concentrations of pepsinogen in a plasma pool.
比较了四种估算血浆胃蛋白酶原浓度的方法，以确定最适合常规使用的方法。其中三种方法是比色测定法，它们使用甘氨酸缓冲白蛋白、白蛋白或血红蛋白作为底物。第四种方法是径向扩散测定法，它使用琼脂糖中的酪蛋白作为底物。当在同一天对重复样品进行检测时，所有四种方法都给出了可重复的结果。然而，第一种方法的日间差异最小，并且是唯一一种与血浆库中不同浓度胃蛋白酶原呈线性关系的方法。
