Luján L, Begara I, Collie D D, Watt N J
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, UK.
Vet Immunol Immunopathol. 1995 Nov;49(1-2):89-100. doi: 10.1016/0165-2427(95)05460-n.
A study to determine the putative relationship between lymphocyte phenotypic alterations in bronchoalveolar lavage fluid and stage of lung pathology in maedi-visna infected sheep has been carried out. Twenty-one ewes (16 Texel and five Scottish blackface) naturally infected by maedi-visna virus and three Oxford controls were used. Animals were killed, lungs were removed, bronchoalveolar lavage was performed and pathological studies were completed. Blood samples were also obtained from 16 animals. Lymphocytes in both bronchoalveolar lavage and peripheral blood were labelled with monoclonal antibodies against the main T lymphocyte subsets (CD4, CD8, CD5 and gamma delta TCR) in order to perform flow cytometric studies. Three aspects of pathology were studied: lymphoid interstitial pneumonia, lymphoid follicular hyperplasia and smooth muscle hyperplasia. Percentages of CD4+, CD5+, gamma delta + T cells and the value for the CD4+ / CD8+ ratio in bronchoalveolar lavage of maedi-visna infected animals were significantly decreased (P < 0.05) when compared to controls, while percentages of CD8+ lymphocytes were increased in bronchoalveolar lavage of infected sheep and they were very close to being significant (P = 0.07) when compared to controls. Lesions were evaluated and simple least-squares regression tests demonstrated that there were several significant correlations between various lymphocyte subsets and pathological parameters studied in this work. However, when a multiple regression test was applied to the data, it was observed that only the CD8+ T cell subset both in bronchoalveolar lavage and in blood was significantly correlated with severity of lung pathology. It is concluded that CD8+ lymphocytes are key cells in the development of the interstitial reaction and the lymphocytic alveolitis observed in maedi-visna infected ewes and that the CD8+ alveolitis is a parallel feature to the intensity of lung lesions. It is further suggested that the percentage of CD8+ lymphocytes in bronchoalveolar lavage and in blood may act as in vivo indicators of lung pathology in maedi-visna infected sheep.
开展了一项研究，以确定梅迪 - 维斯纳病毒感染绵羊支气管肺泡灌洗液中淋巴细胞表型改变与肺部病理阶段之间的假定关系。使用了21只自然感染梅迪 - 维斯纳病毒的母羊（16只特克塞尔羊和5只苏格兰黑脸羊）以及3只牛津对照羊。对动物实施安乐死后，取出肺部，进行支气管肺泡灌洗，并完成病理研究。还从16只动物身上采集了血样。为了进行流式细胞术研究，用针对主要T淋巴细胞亚群（CD4、CD8、CD5和γδTCR）的单克隆抗体标记支气管肺泡灌洗液和外周血中的淋巴细胞。研究了病理学的三个方面：淋巴间质性肺炎、淋巴滤泡增生和平滑肌增生。与对照相比，梅迪 - 维斯纳病毒感染动物支气管肺泡灌洗液中CD4 +、CD5 +、γδ + T细胞的百分比以及CD4 + / CD8 +比值显著降低（P < 0.05），而感染绵羊支气管肺泡灌洗液中CD8 +淋巴细胞的百分比增加，与对照相比非常接近显著水平（P = 0.07）。对病变进行了评估，简单的最小二乘回归测试表明，本研究中所研究的各种淋巴细胞亚群与病理参数之间存在若干显著相关性。然而，当对数据应用多元回归测试时，观察到只有支气管肺泡灌洗液和血液中的CD8 + T细胞亚群与肺部病理严重程度显著相关。得出的结论是，CD8 +淋巴细胞是梅迪 - 维斯纳病毒感染母羊间质反应和淋巴细胞性肺泡炎发展中的关键细胞，并且CD8 +肺泡炎与肺部病变强度平行。进一步表明，支气管肺泡灌洗液和血液中CD8 +淋巴细胞的百分比可能作为梅迪 - 维斯纳病毒感染绵羊肺部病理的体内指标。
