Cordier G, Guiguen F, Cadoré J L, Cozon G, Jacquier M F, Mornex J F
Laboratoire d'Immunologie et de Biologie Pulmonaire, Lyon, France.
Clin Exp Immunol. 1992 Oct;90(1):18-24. doi: 10.1111/j.1365-2249.1992.tb05825.x.
In order to investigate the contribution of lymphocytes to interstitial lung disease in animals with visna-maedi infection, we studied in parallel bronchoalveolar cells and lung tissue from slaughter-house animals (n = 29) and from colostrum-deprived lambs transtracheally inoculated with field isolates of visna-maedi virus (n = 9) or saline (n = 6). Lymphocyte subpopulations were identified in bronchoalveolar lavage by immunofluorescence and flow cytometry analysis and in lung tissue using indirect immunohistochemistry. In infected animals a lymphocytic alveolitis containing CD4 and CD8 lymphocytes was observed. Peribronchovascular lymphoid nodules comprise mostly CD4 lymphocytes. Alveolar lymphocytes of both subsets displayed increased expression of MHC class II antigens in animals with naturally occurring maedi but not in experimentally infected ones. A sequential process of lymphocyte attraction and activation is likely to occur in vivo as part of the alveolitis.
为了研究淋巴细胞在维斯纳-梅迪病毒感染动物的间质性肺病中的作用，我们同时研究了来自屠宰场动物（n = 29）以及经气管接种维斯纳-梅迪病毒野毒株（n = 9）或生理盐水（n = 6）的初乳缺乏羔羊的支气管肺泡细胞和肺组织。通过免疫荧光和流式细胞术分析在支气管肺泡灌洗中鉴定淋巴细胞亚群，并使用间接免疫组织化学在肺组织中进行鉴定。在感染动物中观察到含有CD4和CD8淋巴细胞的淋巴细胞性肺泡炎。支气管血管周围淋巴结节主要由CD4淋巴细胞组成。在自然发生梅迪病的动物中，两个亚群的肺泡淋巴细胞均显示MHC II类抗原表达增加，但在实验感染的动物中未观察到这种情况。作为肺泡炎的一部分，体内可能会发生淋巴细胞吸引和激活的连续过程。
