Luján L, Begara I, Collie D D, Watt N J
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Clin Exp Immunol. 1993 Feb;91(2):272-6. doi: 10.1111/j.1365-2249.1993.tb05894.x.
A phenotypic analysis of bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) cells in maedi visna virus (MVV)-infected sheep has been performed. The differential cell count in BALF from MVV-infected animals was characterized by a significant increase (P < 0.05) in lymphocytes and neutrophils. Lymphocyte phenotyping in BALF from MVV-infected sheep showed a significant decrease (P < 0.05) of CD4+ cells, a significant increase (P < 0.05) of CD8+ cells and a significant inversion (P < 0.001) of the CD4+/CD8+ ratio. CD5+ lymphocytes were also significantly decreased (P < 0.05). Gamma delta T cells and B cells did not differ significantly when compared with the controls. No correlation was observed between BALF and PB lymphocyte phenotypes. BALF macrophages from MVV-infected animals showed increased MHC class II expression and BALF lymphocytes from the same animals demonstrated up-regulation of LFA-1 and LFA-3 expression. These findings and their relationship with lentiviral pathogenesis are discussed.
对梅迪 - 维斯纳病毒（MVV）感染绵羊的支气管肺泡灌洗液（BALF）和外周血（PB）细胞进行了表型分析。MVV感染动物的BALF中的细胞分类计数特征为淋巴细胞和中性粒细胞显著增加（P < 0.05）。MVV感染绵羊的BALF中的淋巴细胞表型分析显示CD4 +细胞显著减少（P < 0.05），CD8 +细胞显著增加（P < 0.05），且CD4 + / CD8 +比值显著倒置（P < 0.001）。CD5 +淋巴细胞也显著减少（P < 0.05）。与对照组相比，γδ T细胞和B细胞无显著差异。未观察到BALF和PB淋巴细胞表型之间的相关性。MVV感染动物的BALF巨噬细胞显示MHC II类表达增加，同一动物的BALF淋巴细胞显示LFA - 1和LFA - 3表达上调。讨论了这些发现及其与慢病毒发病机制的关系。
