Lasley B L, Lohstroh P, Kuo A, Gold E B, Eskenazi B, Samuels S J, Stewart D R, Overstreet J W
Institute of Toxicology and Environmental Health, School of Medicine, University of California, Davis 95616-8615, USA.
Am J Ind Med. 1995 Dec;28(6):771-81. doi: 10.1002/ajim.4700280611.
Laboratory methods were adapted or developed to analyze approximately 70,000 daily urine samples collected during more than 2,500 menstrual cycles from 448 women working in the semiconductor industry. An immunoenzymometric assay (IEMA) for human chorionic gonadotropin (hCG) was employed for screening cycles in order to optimize laboratory resources and to reduce the number of samples requiring analysis by less efficient methods. The presence of hCG in urine was confirmed by the definitive immunoradiometric assay (IRMA). The screening assay eliminated 78% of cycles from further analysis because there was no evidence of conception. Thirty-eight of 448 cycles identified as having significant levels of hCG with the IEMA were confirmed as hCG positive with the IRMA. HCG-positive cycles were further evaluated by examination of daily diary data and by laboratory assays for ovarian and pituitary hormones. As a result of these evaluations, 17 of the 38 cycles identified by the IRMA as positive for hCG were found to be nonconceptive cycles. These results demonstrate the effectiveness of screening assays for hCG, as well as the importance of using multiple urinary biomarkers for the detection of early fetal loss with daily urine samples.
我们采用或开发了实验室方法，以分析在超过2500个月经周期内从448名半导体行业女性中收集的约70000份每日尿液样本。我们采用人绒毛膜促性腺激素（hCG）免疫酶联分析法（IEMA）来筛选月经周期，以优化实验室资源，并减少需要用效率较低的方法进行分析的样本数量。尿液中hCG的存在通过确定性免疫放射分析法（IRMA）得以证实。由于没有受孕迹象，筛选分析排除了78%的月经周期进行进一步分析。在448个经IEMA鉴定为hCG水平显著的月经周期中，有38个经IRMA确认为hCG阳性。通过检查每日日记数据以及进行卵巢和垂体激素的实验室检测，对hCG阳性的月经周期进行了进一步评估。这些评估结果显示，在经IRMA鉴定为hCG阳性的38个月经周期中，有17个被发现为未受孕周期。这些结果证明了hCG筛选分析的有效性，以及使用多种尿液生物标志物通过每日尿液样本检测早期胎儿丢失的重要性。
