Qiu Q, Overstreet J W, Todd H, Nakajima S T, Stewart D R, Lasley B L
Institute of Toxicology and Environmental Health, University of California, Davis, California 95616, USA.
Environ Health Perspect. 1997 Aug;105(8):862-6. doi: 10.1289/ehp.97105862.
Total concentrations of follicle stimulating hormone (FSH) were evaluated in daily urine samples from conceptive and nonconceptive menstrual cycles by measurement of the FSH beta subunit following treatment of the samples to dissociate the FSH heterodimer. Samples were self-collected by normal subjects during cycles in which daily blood samples also were obtained. Daily blood and urine specimens were collected prospectively from 10 subject in conceptive cycles, which led to normal pregnancies, and from 10 subjects with bilateral tubal ligations to provide control samples form nonconceptive cycles. Mean serum and urinary FSH concentration profiles wer parallel in both groups following ovulation and during he first 9 days of the luteal phase. Mean values for both serum and urinary FSH rose significantly above the postovulatory baseline by 10-12 days following the midcycle luteinizing hormone (LH) peak in nonconceptive cycles, but did not rise at any time following ovulation during conceptive cycles. Following regression analysis of the changing FSH concentration between days 9-14 post-LH surge in conceptive cycles, a slope of </= 0.02 ng FHS/mg creatinine/day was selected as a cutoff point to identify conceptive cycles. There was a high concordance between the day of LH peak in serum and the day of FSH peak in urine. Therefore, in applying the algorithm, the day of FSH peak in urine was used to determine the days for which the FSH slope would be calculated, i.e., days 9-14 post-FSH peak in urine. The sensitivity and specificity of the change in urinary FSH concentrations to detect pregnancy in a different set of 55 cycles were found to 88.9% and 89.3%, respectively. All six cases of early fetal loss in the sample set were correctly identified. These results suggest that urinary FSH can be used as an additional biomarker for the verification of early pregnancy in prospective epidemiological studies in which early fetal loss is a suspected outcome.
通过对样本进行处理以解离促卵泡激素（FSH）异二聚体，然后测量FSHβ亚基，来评估来自有受孕和未受孕月经周期的每日尿液样本中FSH的总浓度。样本由正常受试者在同时采集每日血样的周期内自行收集。前瞻性地收集了10名有受孕周期（最终正常妊娠）受试者的每日血样和尿样，以及10名双侧输卵管结扎受试者的样本作为未受孕周期的对照样本。排卵后及黄体期的前9天，两组的血清和尿FSH浓度均值曲线平行。在未受孕周期中，血清和尿FSH的均值在月经周期中期促黄体生成素（LH）峰值后的10 - 12天显著高于排卵后基线水平，但在受孕周期中排卵后的任何时间均未升高。对受孕周期中LH峰后第9 - 14天FSH浓度变化进行回归分析后，选择斜率≤0.02 ng FSH/ mg肌酐/天作为识别受孕周期的切点。血清中LH峰出现的日期与尿中FSH峰出现的日期高度一致。因此，在应用该算法时，尿中FSH峰出现的日期用于确定计算FSH斜率的日期，即尿中FSH峰后第9 - 14天。在另一组55个周期中，尿FSH浓度变化检测妊娠的敏感性和特异性分别为88.9%和89.3%。样本集中所有6例早期胎儿丢失的情况均被正确识别。这些结果表明，在早期胎儿丢失为可疑结局的前瞻性流行病学研究中，尿FSH可作为验证早期妊娠的额外生物标志物。
Zotero 插件
