Lorenzo P, Illera M J, Illera J C, Illera M
Departamento de Fisiología Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, España.
Anat Histol Embryol. 1995 Jun;24(2):139-44. doi: 10.1111/j.1439-0264.1995.tb00025.x.
In this study, the chronological changes in the meiotic progress of in vitro maturation of bovine oocytes was analysed. Oocytes (n = 1044) were obtained from a local abattoir and were classified into two groups according to the presence or absence of cumulus cells. They were incubated in microdrops (5 per drop) in TCM-199 (control), TCM-199 + 10% fetal calf serum (treatment 2) and TCM-199 + 10% estrous cow serum (treatment 3). Oocytes were fixed and stained at the end of 0, 6, 12, 18, 24 and 30 h of the beginning of in vitro culture, were evaluated according to the nuclear stage of maturation. These results demonstrated that the immature oocytes at the time of collection (0 h) were in the germinal vesicle stage (GV), and that the highest maturation rate was at 24 h of culture in all treatments. Serum treatments enhanced the maturation rates obtained (52.1 and 55.7%) compared to control (serum-free) medium (42.7%; P < 0.05) in cumulus-cell-enclosed oocytes. In denuded oocytes, the maturation rates were lower compared to cumulus-cells-enclosed oocytes in all treatments. In conclusion, meiotic progression of bovine oocytes can be influenced by the inclusion of sera in the maturation media and by the presence of the cumulus cells.
在本研究中，分析了牛卵母细胞体外成熟减数分裂进程的时间变化。从当地屠宰场获取卵母细胞（n = 1044），并根据卵丘细胞的有无分为两组。将它们在含有TCM - 199（对照）、TCM - 199 + 10%胎牛血清（处理2）和TCM - 199 + 10%发情母牛血清（处理3）的微滴（每滴5个）中培养。在体外培养开始后的0、6、12、18、24和30小时结束时，将卵母细胞固定并染色，根据成熟的核阶段进行评估。这些结果表明，采集时（0小时）的未成熟卵母细胞处于生发泡期（GV），并且在所有处理中，培养24小时时成熟率最高。与对照（无血清）培养基（42.7%；P < 0.05）相比，血清处理提高了卵丘细胞包裹的卵母细胞的成熟率（52.1%和55.7%）。在所有处理中，裸卵的成熟率低于卵丘细胞包裹的卵母细胞。总之，牛卵母细胞的减数分裂进程会受到成熟培养基中血清的添加以及卵丘细胞的存在的影响。
