Fujita M, Morooka T, Fujimoto S, Moriya T, Amako K
Department of Bacteriology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan.
Arch Microbiol. 1995 Dec;164(6):444-7. doi: 10.1007/BF02529743.
Chromosomal DNA of 27 strains of Campylobacter fetus was analyzed by Southern blotting with a probe of the conserved region of sapA. The probe hybridized with 23 strains that produced type A lipopolysaccharide. These strains had more than six sapA homologs. In Southern blots of SalI-digested chromosomal DNA separated by pulsed-field gel electrophoresis, one fragment from 19 strains and two fragments from 4 strains hybridized. These data indicate that multiple sapA homologs are localized to a limited region on the chromosomal DNA of C. fetus and thus suggest the possibility of developing a typing system using this method.
用sapA保守区探针通过Southern印迹法分析了27株胎儿弯曲菌的染色体DNA。该探针与23株产生A 型脂多糖的菌株杂交。这些菌株有六个以上的sapA同源物。在脉冲场凝胶电泳分离的经SalI消化的染色体DNA的Southern印迹中，19株菌株的一个片段和4株菌株的两个片段发生了杂交。这些数据表明多个sapA同源物定位于胎儿弯曲菌染色体DNA的有限区域，因此提示了使用该方法开发分型系统的可能性。
