Saha V, Lillington D M, Shelling A N, Chaplin T, Yaspo M L, Ganesan T S, Young B D
Department of Medical Oncology, St. Bartholomew's Hospital, London, United Kingdom.
Genes Chromosomes Cancer. 1995 Nov;14(3):220-2. doi: 10.1002/gcc.2870140311.
Chromosome 6 has been shown to contain at band q27 a minimal region of deletion associated with epithelial ovarian cancers and AF6, a gene disrupted in acute myeloid leukemia with t(6;11)(q27;q23). Using rapid amplification of cDNA ends by polymerase chain reaction, the breakpoint in AF6 was confirmed and a cDNA clone identified. This clone was used as a probe to screen a chromosome 6 cosmid library, and a single cosmid C-109F0645 was isolated. By fluorescence in situ hybridization, C-109F0465 was found to map distal to the critically deleted region associated with ovarian malignancies. AF6 is therefore distinct from and lies telomeric to this region.
已证明6号染色体在q27带含有与上皮性卵巢癌相关的最小缺失区域，以及AF6基因，该基因在伴有t(6;11)(q27;q23)的急性髓细胞白血病中发生破坏。通过聚合酶链反应快速扩增cDNA末端，确认了AF6中的断点并鉴定了一个cDNA克隆。该克隆用作探针筛选6号染色体黏粒文库，分离出单个黏粒C-109F0645。通过荧光原位杂交发现，C-109F0465定位于与卵巢恶性肿瘤相关的关键缺失区域的远端。因此，AF6与该区域不同且位于其端粒侧。
