Thorén P A, Paxton R J, Estoup A
Department of Genetics, Uppsala University, Sweden.
Insect Mol Biol. 1995 Aug;4(3):141-8. doi: 10.1111/j.1365-2583.1995.tb00019.x.
A high number of (GT)n and (CT)n microsatellites were isolated from a partial genomic library of primitively eusocial wasp, Vespula rufa. On average, (GT)n and (CT)n repeats occur in the genome approximately every 8 kb and 2.5 kb respectively. This is the highest density of dinucleotide microsatellites reported in the literature. Predominant categories of repeats are imperfect and compound repeats for (GT)n microsatellites and perfect repeats for (CT)n microsatellites. Comparisons with other Hymenoptera and with mammalian species showed significant differences in the frequency distribution of these categories of repeats. Many (CT)n and (GT)n repeats are surrounded by various types of microsatellites, suggesting an associative distribution of short tandem repeats. A high level of intracolonial polymorphism is demonstrated with one microsatellite locus, showing that multiple mating of the queen occurs in V. rufa.
从原始群居黄蜂红胡蜂的部分基因组文库中分离出大量的(GT)n和(CT)n微卫星。平均而言，(GT)n和(CT)n重复序列在基因组中分别大约每8 kb和2.5 kb出现一次。这是文献中报道的二核苷酸微卫星的最高密度。重复序列的主要类别是(GT)n微卫星的不完全和复合重复以及(CT)n微卫星的完美重复。与其他膜翅目昆虫和哺乳动物物种的比较显示，这些重复类别在频率分布上存在显著差异。许多(CT)n和(GT)n重复序列被各种类型的微卫星所包围，表明短串联重复序列存在关联分布。一个微卫星位点显示出高水平的群体内多态性，表明红胡蜂中蜂王发生多次交配。
