Estoup A, Solignac M, Harry M, Cornuet J M
Laboratoire de Biologie et Génétique Evolutives, CNRS, France.
Nucleic Acids Res. 1993 Mar 25;21(6):1427-31. doi: 10.1093/nar/21.6.1427.
A set of 52 (CT)n and 23 (GT)n microsatellites in honeybee, 24 (CT)n and 2 (GT)n microsatellites in bumble-bee (n > 6) have been isolated from partial genomic libraries and sequenced. On average, (CT)n and (GT)n microsatellites occur every 15 kb and 34 kb in honeybee and every 40 kb and 500 kb in bumble-bee, respectively. The prevailing categories are imperfect repeats for (CT)n microsatellites in bumble-bee, and perfect repeats for both (CT)n and (GT)n microsatellites in honey-bee. Comparisons with data available in vertebrates indicate a lower proportion of perfect repeats in bees but length distributions are very similar regardless the phylum. This result extends to insects the concept of an evolutionary conservation for quantitative and qualitative characteristics of (CT)n and (GT)n microsatellites. Many (CT)n and (GT)n repeats are surrounded with various types of microsatellites, revealing an associative distribution of short repeat sequences. As expected, a high level of intrapopulational polymorphism has been found with one tested honeybee microsatellite. Also, flanking regions of this microsatellite are similar enough to allow PCR amplification in several other species of Apis and Bombus.
从部分基因组文库中分离并测序了蜜蜂中的一组52个(CT)n和23个(GT)n微卫星，以及熊蜂中的24个(CT)n和2个(GT)n微卫星(n>6)。平均而言，(CT)n和(GT)n微卫星在蜜蜂中分别每15 kb和34 kb出现一次，在熊蜂中分别每40 kb和500 kb出现一次。熊蜂中(CT)n微卫星的主要类别是不完全重复，而蜜蜂中(CT)n和(GT)n微卫星的主要类别都是完全重复。与脊椎动物的现有数据比较表明，蜜蜂中完全重复的比例较低，但无论门类如何，长度分布都非常相似。这一结果将(CT)n和(GT)n微卫星数量和质量特征的进化保守概念扩展到了昆虫。许多(CT)n和(GT)n重复序列周围都有各种类型的微卫星，揭示了短重复序列的关联分布。正如预期的那样，在一个测试的蜜蜂微卫星中发现了高水平的种群内多态性。此外，该微卫星的侧翼区域足够相似，能够在其他几种蜜蜂和熊蜂物种中进行PCR扩增。
