El-Aaser A A, El-Merzabani M M
Z Klin Chem Klin Biochem. 1975 Oct;13(10):453-9. doi: 10.1515/cclm.1975.13.10.453.
A simple method is described for the simultaneous determination of alkaline phosphatase (EC 3.1.3.1) and 5'-nucleotidase (EC 3.1.3.5) in serum. The method is based on the determination of inorganic phosphorus released by the action of the two enzymes on adenosine-5'-monophosphate at pH 9.5 (200 mmol/l tris-buffer) in the presence and absence of L-cysteine. This amino acid at a concentration of 2--10 mmol/l was found to be a specific inhibitor for alkaline phosphatase but with no effect on 5'-nucleotidase activity.
本文描述了一种同时测定血清中碱性磷酸酶（EC 3.1.3.1）和5'-核苷酸酶（EC 3.1.3.5）的简单方法。该方法基于在pH 9.5（200 mmol/l三羟甲基氨基甲烷缓冲液）条件下，在有和没有L-半胱氨酸存在的情况下，测定两种酶作用于5'-单磷酸腺苷所释放的无机磷。发现浓度为2 - 10 mmol/l的这种氨基酸是碱性磷酸酶的特异性抑制剂，但对5'-核苷酸酶活性没有影响。
