Baginski E S, Marie S S, Epstein E, Zak B
Ann Clin Lab Sci. 1977 Nov-Dec;7(6):469-78.
A simple determination of 5'-Nucleotidase in blood serum without deproteinization is described. The enzyme activity is distinguished from that of a nonspecific alkaline phosphatase by nickel inhibition and the inorganic phosphate released from adenosine monophosphate used as substrate is determined by a method previously described. Additional studies include the determination of optimal conditions for the reaction and nickel inhibition.
本文描述了一种无需脱蛋白即可简单测定血清中5'-核苷酸酶的方法。通过镍抑制作用将该酶活性与非特异性碱性磷酸酶的活性区分开来，并且采用先前描述的方法测定用作底物的一磷酸腺苷释放的无机磷酸盐。额外的研究包括确定反应的最佳条件和镍抑制作用。
