Blecker U, Lanciers S, Hauser B, de Pont S M, Vandenplas Y
Division of Paediatric Gastroenterology, Academic Children's Hospital, Free University of Brussels, Belgium.
Eur J Gastroenterol Hepatol. 1995 Oct;7(10):979-83. doi: 10.1097/00042737-199510000-00013.
To evaluate the diagnostic value of specific immunoglobulins G and M in the detection of Helicobacter pylori infection.
Sera from 68 H. pylori-infected children and 35 H. pylori-negative children (aged 1-15 years) were examined using recent-generation serum enzyme-linked immunosorbent assay (ELISA) tests (Malakit Helicobacter pylori series; Biolab, Limal, Belgium).
All of the investigated children had positive values for immunoglobulin G antibodies. However, anti-H. pylori-specific immunoglobulin M could be detected only in 19 (27.9%) of the 68 children. When compared with the 13C-urea breath test, these findings represent sensitivity, specificity and positive and negative predictive values of 27.9, 100, 100 and 41.7%, respectively, for the serum ELISA test for the detection of H. pylori-specific immunoglobulin M compared with 100.0, 97.2, 98.5 and 100.0%, respectively, for the Malakit for the detection of specific immunoglobulin G antibodies.
Although H. pylori-specific immunoglobulin G has been established as a reliable indicator of active H. pylori infection, using the Malakit for the detection of H. pylori-specific immunoglobulin M does not seem to be of major value in the diagnosis of H. pylori infection in children.
评估特异性免疫球蛋白G和M在检测幽门螺杆菌感染中的诊断价值。
采用新一代血清酶联免疫吸附测定（ELISA）检测（Malakit幽门螺杆菌系列；比利时利马尔市Biolab公司）68例幽门螺杆菌感染儿童和35例幽门螺杆菌阴性儿童（年龄1至15岁）的血清。
所有受调查儿童的免疫球蛋白G抗体值均为阳性。然而，68例儿童中仅19例（27.9%）可检测到抗幽门螺杆菌特异性免疫球蛋白M。与13C尿素呼气试验相比，血清ELISA检测幽门螺杆菌特异性免疫球蛋白M的敏感性、特异性、阳性预测值和阴性预测值分别为27.9%、100%、100%和41.7%，而Malakit检测特异性免疫球蛋白G抗体的相应值分别为100.0%、97.2%、98.5%和100.0%。
虽然幽门螺杆菌特异性免疫球蛋白G已被确立为活动性幽门螺杆菌感染的可靠指标，但使用Malakit检测幽门螺杆菌特异性免疫球蛋白M在儿童幽门螺杆菌感染诊断中似乎没有太大价值。
