Kitaura H, Ohara N, Matsuo T, Yamada T
School of Dentistry, Nagasaki University, Japan.
New Microbiol. 1995 Oct;18(4):429-33.
The internal control of DNA for the rapid detection of mycobacteria by PCR is described. The 1100bp fragment for internal control was produced from Streptomyces lividans DNA with the primers used for the rapid detection of mycobacteria by PCR. The amplified reaction consequently produced two products with 782bp for mycobacteria and 1100bp for the internal control extracted from all mycobacterial DNAs containing internal control so far examined. The 1100bp amplified fragment proved to be useful as an internal control with the same primer-binding sequence for the detection of mycobacteria.
本文描述了用于通过PCR快速检测分枝杆菌的DNA内部控制。用于内部控制的1100bp片段是从淡紫链霉菌DNA中产生的，使用的引物是用于通过PCR快速检测分枝杆菌的。因此，扩增反应从迄今为止检测的所有含有内部控制的分枝杆菌DNA中产生了两种产物，一种是782bp的分枝杆菌产物，另一种是1100bp的内部控制产物。事实证明，1100bp的扩增片段作为具有相同引物结合序列的内部控制，可用于分枝杆菌的检测。
