Lepistö R, Rintala J A
Institute of Water and Environmental Engineering, Tampere University of Technology, Finland.
Appl Microbiol Biotechnol. 1995 Nov;43(6):1001-5. doi: 10.1007/BF00166916.
This study focused on the use the thermophilic anaerobic granulae in the start-up of 70 degrees C acetate-fed upflow anaerobic sludge-blanket (UASB) reactors and the kinetics of granulae grown at 70 degrees C. In the UASB reactors, chemical oxygen demand removal commenced within 48 h of the start-up. The maximum reduction in chemical oxygen demand was 84% with the feed containing yeast and 71% without a yeast supplement. In the bioassays, the yeast-grown sludge converted 98% of the acetate consumed to methane as compared to 92% for the sludge grown without yeast. The highest initial specific methane production rate (mu-CH4) of the UASB sludges grown at 70 degrees C was 0.088 h(-1) at an acetate concentration of 4.6mM. The higher initial acetate concentration was found to prolong the lag-phase in methane production significantly and to decrease mu-CH4. The half-saturation constant (Ks), the inhibition constant (Ki), the inhibition response coefficient (n) and the mu-CH4-max, calculated according to a modified Haldane equation, were 1.5 mM, 2.8 mM, 0.8 and 0.28 h(-1), respectively. The prolonged starvation of the 70 degrees C sludge (15 days) decreased the mu-CH4 from about 0.022 h(-1) to 0.011 h(-1) and increased the lag phase in methane production from 6 h to 24 h as compared with non-starved sludge.
本研究聚焦于嗜热厌氧颗粒在70摄氏度以乙酸盐为进料的上流式厌氧污泥床（UASB）反应器启动过程中的应用以及在70摄氏度下生长的颗粒的动力学。在UASB反应器中，启动后48小时内化学需氧量去除即开始。含酵母进料时化学需氧量的最大降幅为84%，不含酵母补充剂时为71%。在生物测定中，酵母培养的污泥将消耗的乙酸盐的98%转化为甲烷，而无酵母培养的污泥这一比例为92%。70摄氏度下生长的UASB污泥的最高初始比甲烷产生速率（μ-CH4）在乙酸盐浓度为4.6mM时为0.088 h-1。发现较高的初始乙酸盐浓度会显著延长甲烷产生的滞后期并降低μ-CH4。根据修正的哈代方程计算的半饱和常数（Ks）、抑制常数（Ki）、抑制响应系数（n）和μ-CH4-max分别为1.5 mM、2.8 mM、0.8和0.28 h-1。与未饥饿的污泥相比，70摄氏度污泥的长期饥饿（15天）使μ-CH4从约0.022 h-1降至0.011 h-1，并使甲烷产生的滞后期从6小时增加到24小时。
