Water and Environmental Engineering, Tampere University of Technology, P.O. Box 600, SF-33101 Tampere, Finland, and The Anaerobic Microbiology/Biotechnology Group, Department of Biotechnology, The Technical University of Denmark, DK-2800 Lyngby, Denmark.
Appl Environ Microbiol. 1993 Jun;59(6):1742-6. doi: 10.1128/aem.59.6.1742-1746.1993.
Anaerobic acetate degradation at 70 degrees C and at 55 degrees C (as a reference) was studied by running laboratory upflow anaerobic sludge blanket (UASB) reactors inoculated with mesophilic granular sludge. In UASB reactors fed with acetate-containing media (3 g of chemical oxygen demand [COD] per liter, corresponding to 47 mM acetate) approximately 50 days was needed at 70 degrees C and less than 15 days was needed at 55 degrees C to achieve an effluent COD of 500 to 700 mg/liter. In the UASB reactors at both 70 and 55 degrees C up to 90% of the COD was removed. Batch assays showed that sludges from two 70 degrees C UASB reactors, one run at a low effluent acetate concentration and the other run at a high effluent acetate concentration, exhibited slightly different responses to temperatures in the range from 37 to 70 degrees C. Both 70 degrees C sludges, as well as the 55 degrees C sludge, produced methane at temperatures of 37 to 73 degrees C. The 55 degrees C sludge exhibited shorter lag phases than the 70 degrees C sludges and higher specific methane production rates between 37 and 65 degrees C.
采用接种中温颗粒污泥的实验室上流式厌氧污泥床（UASB）反应器，研究了在 70℃和 55℃（作为参照）下的厌氧乙酸盐降解。在 UASB 反应器中，以含有乙酸盐的培养基为食（每升化学需氧量为 3 克，相当于 47mM 乙酸盐），在 70℃下需要大约 50 天，在 55℃下需要不到 15 天，即可使出水 COD 达到 500 至 700mg/L。在 70 和 55℃的 UASB 反应器中，高达 90%的 COD 被去除。批处理试验表明，来自两个 70℃ UASB 反应器的污泥，一个在低出口乙酸盐浓度下运行，另一个在高出口乙酸盐浓度下运行，对 37 至 70℃范围内的温度表现出略有不同的响应。在 37 至 73℃的温度下，两个 70℃的污泥以及 55℃的污泥均产生甲烷。55℃的污泥比 70℃的污泥表现出更短的迟滞期，并且在 37 至 65℃之间具有更高的特定甲烷生成速率。
