Van Belle K, Verfaillie I, Ebinger G, Michotte Y
Department of Pharmaceutical Chemistry and Drug Analysis, Vrije Universiteit Brussels, Belgium.
J Chromatogr B Biomed Appl. 1995 Oct 6;672(1):97-102. doi: 10.1016/0378-4347(95)00195-o.
An isocratic LC assay using a microcolumn (800 microns I.D.) coupled to a U-shaped optical flow cell (cell volume 70 nl; optical path length 8 mm) for high-sensitivity UV absorbance is described for the detection of oxcarbazepine and its major and active metabolite, 10,11-dihydro-10-hydroxycarbamazepine in microdialysates. Using the combination microcolumn-capillary UV detector, a ten-fold increase in sensitivity was obtained resulting in a limit of detection of 10 pg/10 microliters. This assay is sufficiently sensitive to allow quantification of drug and metabolite in 10-microliters aliquots of rat blood and hippocampus microdialysates, using carbamazepine-10,11-epoxide as external standard.
本文描述了一种等度液相色谱分析法，该方法使用内径为800微米的微柱与U形光流通池（池体积70纳升；光程长度8毫米）相连，用于高灵敏度紫外吸光度检测，以检测微透析液中的奥卡西平及其主要活性代谢物10,11-二氢-10-羟基卡马西平。使用微柱-毛细管紫外检测器组合，灵敏度提高了10倍，检测限达到10皮克/10微升。该分析方法灵敏度足够高，以卡马西平-10,11-环氧化物为外标，能够对10微升大鼠血液和海马微透析液中的药物和代谢物进行定量分析。
