Immunocytochemical study of type IV collagen and laminin in canine mammary tumours.

We performed an immunohistochemical study of the basement membrane (BM) proteins laminin and type IV collagen in 35 canine mammary tumours, histologically classified according to the World Health Organization's suggested classification. Laminin and type IV collagen were seen in extracellular locations but only laminin was present in epithelial and myoepithelial cells and chondrocytes. The BM was always continuous in adenomas and fibroadenomas. In the 20 malignant tumours studied, the basement membrane was continuous in one, discontinuous in five, and virtually absent in 14. When present, the BM of malignant tumours varied in width and distribution in the same area. The benign and malignant mixed tumours did not have BM separating epithelial and newly-formed mesenchymal structures. In areas of abundant myoepithelial cells in complex and mixed tumours, both benign and malignant, there were groups of BM fragments which differed in length and thickness and occasionally formed tangles. We have called these 'membranous thickets'. Our results indicate that myoepithelial cells are the main source of the BM proteins laminin and type IV collagen in canine mammary tumours.