Grund S, Nattermann H, Horsch F
Institut für Mikrobiologie und Tierseuchen/Elektronenmikroskopie, Fachbereich Veterinärmedizin der Freien Universität Berlin, Deutschland.
Zentralbl Veterinarmed B. 1995 Nov;42(9):533-42.
In typical Dermatitis digitalis (D.d.) lesions, spirochaete-like bacteria with variations in spiralization were revealed by electron microscopy. While, in the early stages of the disease, these are found to be associated with fibrillar material of keratocytes, they occur massively in vacuoles at more advanced stages. The spirochaetes carry one pair of endoflagella, originating with a hook from the poles of the bacteria. These flagellae are composed of coiled flagellating fibrils in the pole region, merging towards the centre of the bacterium. A coat of fibrils was found in association with the cytoplasmatic membrane. The winding of this coat follows and may influence the coiling of the protoplast, and is probably involved in the rapid motility of this spirochaetes, together with the flagella. Immuno-electronmicroscopy revealed an antigenic relationship with Borrelia burgdorferi, at least with regard to the regions of flagella and undulating membrane. The paper discusses: 1. The possible classification of these spirochaetes with the genus Treponema; 2. The layer of peptidoglycan occurring on the outer membrane; and 3. The keratolytic activity of spirochaetes in D.d.
在典型的指部皮炎（D.d.）病变中，通过电子显微镜发现了具有螺旋化变化的螺旋体样细菌。在疾病早期，这些细菌与角质形成细胞的纤维状物质相关，而在疾病更晚期则大量出现在液泡中。这些螺旋体带有一对内鞭毛，从细菌的极部以钩状起始。这些鞭毛由极部区域的盘绕鞭毛纤维组成，向细菌中心汇聚。发现一层纤维与细胞质膜相关。这层纤维的缠绕跟随并可能影响原生质体的盘绕，并且可能与这些螺旋体与鞭毛一起的快速运动有关。免疫电子显微镜显示与伯氏疏螺旋体存在抗原关系，至少在鞭毛和波动膜区域是这样。本文讨论了：1. 这些螺旋体与密螺旋体属的可能分类；2. 在外膜上出现的肽聚糖层；以及3. 螺旋体在指部皮炎中的角质溶解活性。
