Döpfer D, Koopmans A, Meijer F A, Szakáll I, Schukken Y H, Klee W, Bosma R B, Cornelisse J L, van Asten A J, ter Huurne A A
Department of Infectious Diseases and Immunology, Utrecht University, The Netherlands.
Vet Rec. 1997 Jun 14;140(24):620-3. doi: 10.1136/vr.140.24.620.
Tissue samples from the feet of slaughtered cattle exhibiting different stages of digital dermatitis were sectioned and stained with haematoxylin and eosin and silver staining techniques. Three morphological variations of spirochaetes were observed, whereas control samples from feet which were macroscopically negative for digital dermatitis were also negative for spirochaetes. In an immunofluorescence test, Campylobacter faecalis was found to be abundant on superficial wound smears from the classical ulceration of digital dermatitis.
对呈现不同阶段指皮炎的屠宰牛足部组织样本进行切片，并采用苏木精-伊红染色和银染色技术染色。观察到螺旋体有三种形态变化，而指皮炎肉眼检查呈阴性的足部对照样本螺旋体检测也呈阴性。在免疫荧光试验中，发现粪弯曲菌在指皮炎典型溃疡的浅表伤口涂片上大量存在。
