Lee C S, Pirdas A, Lee M W
Department of Pathology, University of Melbourne.
Pathology. 1996 Aug;28(3):220-4. doi: 10.1080/00313029600169014.
Expression of the 17kD product of the human nm23-H1 gene has been shown to be reduced in a number of human malignancies including those from the breast, colon, kidney and lung. Somatic allelic deletion of nm23-H1 gene is also associated with a high incidence of distant metastasis and reduced patient survival in colorectal and infiltrating breast ductal carcinomas. We compared the immunohistochemical reactivity of the nm23-H1 gene product in the different histological subtypes of human cutaneous melanoma to that of Spitz nevi. All nine cases of Spitz nevi and five cases of in situ melanoma showed diffuse and intense nm23-H1 protein immunoreactivity. The majority of superficial spreading melanomas (18 of 27 cases) had moderate to strong nm23-H1 protein immunostaining. In contrast, the majority of nodular melanomas (7 of 8 cases) with poor prognostic histological indices displayed marked reduction in immunoreactivity to the nm23-H1 protein. The 2 cases of metastatic melanoma showed reduced or no nm23-H1 protein immunostaining. In conclusion, reduced nm23-H1 immunohistological expression is associated with melanomas that have high metastatic potential and poorer prognosis.
已证实，人类nm23-H1基因的17kD产物在包括乳腺癌、结肠癌、肾癌和肺癌在内的多种人类恶性肿瘤中表达降低。nm23-H1基因的体细胞等位基因缺失也与结直肠癌和浸润性乳腺导管癌远处转移的高发生率及患者生存率降低有关。我们比较了人类皮肤黑色素瘤不同组织学亚型中nm23-H1基因产物与Spitz痣的免疫组化反应性。所有9例Spitz痣和5例原位黑色素瘤均显示nm23-H1蛋白弥漫性强免疫反应性。大多数浅表扩散性黑色素瘤（27例中的18例）具有中度至强的nm23-H1蛋白免疫染色。相比之下，大多数具有不良预后组织学指标的结节性黑色素瘤（8例中的7例）对nm23-H1蛋白的免疫反应性明显降低。2例转移性黑色素瘤显示nm23-H1蛋白免疫染色降低或无染色。总之，nm23-H1免疫组化表达降低与具有高转移潜能和较差预后的黑色素瘤有关。
