Elschner M
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Fachbereich 4 Bakterielle Tierseuchen und Bekämpfung von Zoonosen, Jena.
Berl Munch Tierarztl Wochenschr. 1995 Jul;108(7):256-60.
A haemagglutination assay was tested to detect bovine parvoviruses (BPV) in 456 faecal samples of calves. The assay was not suitable because of a high number of nonspecific haemagglutinating reactions and many haemolytical specimens. A 16fold higher sensitivity was obtained using solid-phase immune electronmicroscopy (SPIEM) in comparison with direct negative contrast staining for detection of BPV. Therefore we examined 117 faecal specimens from young calves of dairy herds from the eastern part of Thuringia by SPIEM. We detected BPV in 2 faecal specimens of calves with diarrhoea and in 3 faecal specimens of clinically normal calves. In comparison with results of other investigators this detection rate of BPV was relatively high.
采用血凝试验检测456份犊牛粪便样本中的牛细小病毒（BPV）。由于非特异性血凝反应数量众多且有许多溶血样本，该试验并不适用。与直接负染法相比，固相免疫电子显微镜（SPIEM）检测BPV的灵敏度高16倍。因此，我们采用SPIEM检查了图林根州东部奶牛场117份犊牛粪便样本。在2份腹泻犊牛粪便样本和3份临床正常犊牛粪便样本中检测到了BPV。与其他研究人员的结果相比，BPV的检出率相对较高。
