Fujii T, Ha H, Yokoyama H, Hamamoto H, Yoon S H, Hori H
Tag Biosystem Institute, Tokushima, Japan.
Biol Pharm Bull. 1995 Oct;18(10):1446-9. doi: 10.1248/bpb.18.1446.
We investigated the optimal conditions for the application of the MTT assay to determine the viability of cell in primary cultures of rat hepatocytes. The optimal conditions were found to be: inoculation at cell density of 1.31 x 10(5) cells/cm2; concentration of 3-(4,5-dimethylthiazoyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 0.12 mM; and MTT incorporation time, 2 h or more. We also found that insulin and glucocorticoid, which are usually added to the medium to maintain cell function, had no effect on the viability of the primary cultures of rat hepatocytes.
我们研究了应用MTT法测定大鼠原代肝细胞培养物中细胞活力的最佳条件。发现最佳条件为：以1.31×10⁵个细胞/cm²的细胞密度接种；3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）浓度为0.12 mM；MTT掺入时间为2小时或更长时间。我们还发现，通常添加到培养基中以维持细胞功能的胰岛素和糖皮质激素对大鼠原代肝细胞培养物的活力没有影响。
