Rump A F, Klaus W
Institut für Pharmakologie, Universität Köln, Cologne, Germany.
Arzneimittelforschung. 1995 Oct;45(10):1063-6.
The effects of exogenous superoxide dismutase (SOD) on acute myocardial ischemia (MI) was investigated in isolated electrically-driven rabbit hearts (Langendorff, constant pressure: 70 cm H2O, Tyrode solution, Ca++ 1.8 mmol/l, 37 degrees C). Acute regional ischemia (MI) was induced by occlusion of a coronary artery branch (CAO) and quantitated from epicardial NADH-fluorescence photography. SOD (48 U/ml) was applied either 30 min after CAO in a single coronary occlusion model (treatment) or 30 min before the 2nd CAO in a repetitive coronary occlusion model (pre-treatment). SOD had no significant influence on the left ventricular pressure or the global coronary flow (p > 0.05). MI was significantly diminished in hearts pre-treated with SOD before CAO (-25%)(p < 0.05), but remained unaffected when SOD was applied after CAO (p > 0.05). The results suggest that superoxide anion radicals contribute to ischemic tissue injury. SOD shows cardioprotective properties only if present in the ischemic zone, requiring the application of SOD before CAO in poorly collateralised rabbit hearts.
在离体电驱动兔心脏（Langendorff 装置，恒压：70 cm H₂O，台氏液，Ca²⁺ 1.8 mmol/L，37℃）中研究了外源性超氧化物歧化酶（SOD）对急性心肌缺血（MI）的影响。通过冠状动脉分支闭塞（CAO）诱导急性局部缺血（MI），并从心外膜 NADH 荧光摄影进行定量分析。在单次冠状动脉闭塞模型中，SOD（48 U/ml）在 CAO 后 30 分钟应用（治疗组）；在重复冠状动脉闭塞模型中，SOD 在第二次 CAO 前 30 分钟应用（预处理组）。SOD 对左心室压力或整体冠状动脉血流无显著影响（p > 0.05）。在 CAO 前用 SOD 预处理的心脏中，MI 显著减轻（-25%）（p < 0.05），但在 CAO 后应用 SOD 时 MI 无变化（p > 0.05）。结果表明，超氧阴离子自由基参与缺血性组织损伤。SOD 仅在缺血区存在时才显示出心脏保护特性，这要求在侧支循环不良的兔心脏中在 CAO 前应用 SOD。
