Rump A F, Schierholz J M, Klaus W
Department of Pharmacology, University of Cologne, Germany.
Arzneimittelforschung. 1998 Nov;48(11):1078-82.
Ascorbic acid (CAS 50-81-7) might mediate cardioprotective effects by scavenging free oxygen radicals. The effects of exogenous ascorbic acid on acute myocardial ischemia (MI) was investigated in isolated electrically-driven rabbit hearts (Langendorff, constant pressure: 70 cm H2O, Tyrode solution, Ca2+ 1.8 mmol/l, 37 degrees C). Repetitive MI, separated by a reperfusion period of 50 min, was induced by coronary artery branch ligature and quantitated from epicardial NADH-fluorescence photography. Starting after a reperfusion period of 20 min, isolated hearts were treated with ascorbic acid (10(-5) or 10(-4) mol/l). Ascorbic acid had no significant influence on the left ventricular left ventricular pressure or the coronary flow (p > 0.05). Ascorbic acid had no significant effect on epicardial NADH-fluorescence area or intensity (p > 0.05). Free radical scavenging properties reported for ascorbic acid do not mediate cardioprotective effects at the concentrations used in isolated rabbit hearts.
抗坏血酸（CAS 50-81-7）可能通过清除氧自由基来介导心脏保护作用。在离体电驱动兔心脏（Langendorff装置，恒压：70 cm H₂O，台氏液，Ca²⁺ 1.8 mmol/L，37℃）中研究了外源性抗坏血酸对急性心肌缺血（MI）的影响。通过冠状动脉分支结扎诱导重复性MI，每次MI间隔50分钟的再灌注期，并通过心外膜NADH荧光摄影进行定量分析。在20分钟的再灌注期后开始，对离体心脏用抗坏血酸（10⁻⁵或10⁻⁴ mol/L）进行处理。抗坏血酸对左心室压力或冠状动脉流量无显著影响（p＞0.05）。抗坏血酸对心外膜NADH荧光面积或强度无显著影响（p＞0.05）。在离体兔心脏中使用的浓度下，抗坏血酸所报道的自由基清除特性并未介导心脏保护作用。
