Kaytor M D, Livingston D M
Department of Biochemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Curr Genet. 1996 Feb;29(3):203-10. doi: 10.1007/BF02221549.
We screened for rad52 suppressors against temperature-sensitive (ts), missense, nonsense, and deletion rad52 mutations. Except for the deletion strain all mutants yielded suppressor candidates, indicating that suppressors completely bypassing the need for RAD52 are rare. Characterization of seven, recessive extragenic suppressors from our screen and two previously identified suppressors revealed that nearly all exhibit allele specificity. The allele specificity is positional in that suppressors that suppress a ts mutation in the C-terminal third of the coding region do not suppress three ts mutations in the N-terminal third. Conversely, suppressors against one of the three N-terminal mutations suppress more than one of these mutations but not the C-terminal mutation.
我们筛选了针对温度敏感型（ts）、错义、无义及缺失型rad52突变的rad52抑制子。除缺失菌株外，所有突变体均产生了抑制子候选物，这表明完全绕过对RAD52需求的抑制子很罕见。对我们筛选出的7个隐性基因外抑制子和2个先前鉴定的抑制子进行表征后发现，几乎所有抑制子都表现出等位基因特异性。这种等位基因特异性具有位置相关性，即抑制编码区C端三分之一处ts突变的抑制子不会抑制N端三分之一处的三个ts突变。相反，针对三个N端突变之一的抑制子能抑制这些突变中的不止一个，但不能抑制C端突变。
