Calonge M L, Ilundáin A A
Departamento Fisiología y Biología Animal, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain.
Pflugers Arch. 1996 Feb;431(4):594-8. doi: 10.1007/BF02191908.
Chicken enterocytes present a H+-conducting pathway involved in the recovery of intracellular pH (pHi) from an acid load. In the current study we have tested the effect of protein kinase C (PKC) activators on the rate of proton efflux through the H+-conducting pathway. The rate of proton efflux was increased by the addition of 1,2-dioctanoyl-rac-glycerol (DOG) or phorbol 12-myristate 13-acetate (PMA), but it was not affected by the addition of the inactive phorbol ester analogue, 4alpha-phorbol 12, 13-didecanoate. DOG stimulated the process in a dose-dependent manner with a half-maximal effect at 45 microM. Staurosporine and Zn2+ prevented the DOG-dependent increase in the rate of proton efflux. The rate of proton efflux was affected by the pH, and DOG shifted this relationship upward and to the right. These results suggest that the proton-conducting pathway is regulated by PKC.
鸡小肠上皮细胞存在一条参与从酸负荷中恢复细胞内pH（pHi）的H⁺传导途径。在本研究中，我们测试了蛋白激酶C（PKC）激活剂对通过H⁺传导途径的质子外流速率的影响。添加1,2 - 二辛酰 - rac - 甘油（DOG）或佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）可增加质子外流速率，但添加无活性的佛波醇酯类似物4α - 佛波醇12,13 - 二癸酸酯对其无影响。DOG以剂量依赖方式刺激该过程，在45 μM时达到半数最大效应。星形孢菌素和Zn²⁺可阻止DOG依赖的质子外流速率增加。质子外流速率受pH影响，DOG使这种关系向上和向右移动。这些结果表明质子传导途径受PKC调节。
