Atchison D J, Johnston M G
Department of Pathology, University of Toronto, Canada.
Pflugers Arch. 1996 Feb;431(4):618-24. doi: 10.1007/BF02191911.
We investigated the hypothesis that atrial natriuretic peptide (ANP) attenuates lymph vessel pumping. In the present experiments, isolated bovine lymphatic vessels were cannulated at each end to create inflow and outlfow ports for the administration of Krebs' solution (vehicle) or ANP and for the measurement of fluid pumped by the vessel respectively. Once cannulated, the vessels were placed in a temperature-regulated bath circulated with oxygenated vehicle. Transmural pressure was regulated by the height of a fluid-filled reservoir. Lymph pump activity was assessed by measuring the volume of outlfow every ten minutes, ANP was administered at concentrations of 0 (control), 0.1, 1.0, 10 and 100 nM. Data were expressed as a percentage of the value in the control period. When compared with vehicle, ANP produced a significant inhibition of lymph pump activity by 7.7% at 0.1 nM, 24.2% at 10 nM and 38.26% at 100 nM averaged over the hour for which the vessels were exposed to each concentration. Thus ANP inhibits lymph pumping concentration dependently. This may be yet another mechanism by which ANP exerts its haemodynamic effects.
我们研究了心房利钠肽（ANP）减弱淋巴管泵血功能这一假说。在本实验中，将分离的牛淋巴管两端插管，分别用于注入 Krebs 溶液（溶剂）或 ANP 以及测量淋巴管泵出的液体量，从而形成流入端口和流出端口。插管后，将淋巴管置于用含氧溶剂循环的温度调节浴中。通过充满液体的储液器高度调节跨壁压力。每十分钟测量流出量来评估淋巴泵活动，分别以 0（对照）、0.1、1.0、10 和 100 nM 的浓度给予 ANP。数据表示为对照期值的百分比。与溶剂相比，在淋巴管暴露于每种浓度的一小时内，平均而言，ANP 在 0.1 nM 时使淋巴泵活动显著抑制 7.7%，在 10 nM 时抑制 24.2%，在 100 nM 时抑制 38.26%。因此，ANP 浓度依赖性地抑制淋巴泵血。这可能是 ANP 发挥其血液动力学作用的又一机制。
