Duffaud F, Favre R, Botta A
Service d'oncologie médicale, CHU Timone-adultes, Marseille, France.
Ann Biol Clin (Paris). 1995;53(7-8):435-8.
In spite of the development of new automated methods proposed for scoring of micronuclei, manual analysis of micronucleated cells by light microscopy is still largely employed. The purpose of this study was to define the minimal number of mononucleated and binucleated cells representative of the total number of cells of the side, to determine the division rate. The second aim was to define the minimal number of binucleated cells representative of the total number of the binucleated cells on the same slide, to determine the rate of binucleated micronucleated cells. Total mononucleated, binucleated and polynucleated cells were scored in each slide for determining the 'theoretical division rate'. Among total binucleated lymphocytes observed in each slide, micronucleated cells containing one, two or more micronuclei were scored to determine the 'theoretical micronuclei rate'. Then, 'experimental division rates' were calculated by dividing microscope slides into random areas of 500 lymphocytes, and recording mononucleated, binucleated cells. 'Experimental micronuclei rates' were determined by dividing microscope slides into random areas of 500 binucleated cells, and recording micronucleated cells containing one or more micronuclei. 'Experimental division rates' obtained by examining sets of 4000 cells were shown to be not representative of the corresponding 'theoretical rate' (statistical difference by the chi-squared test, p < 0.05)). 'Experimental micronuclei rates' obtained by examining sets of 500 binucleated cells were shown to be representative of the corresponding 'theoretical micronuclei rate' (no statistical difference by the chi-squared test, p > 0.05).
尽管已经开发出用于微核评分的新型自动化方法，但通过光学显微镜对微核化细胞进行手动分析仍被广泛采用。本研究的目的是确定代表一侧细胞总数的单核细胞和双核细胞的最少数量，以确定分裂率。第二个目的是确定代表同一张载玻片上双核细胞总数的双核细胞的最少数量，以确定双核微核细胞的比率。对每张载玻片上的总单核、双核和多核细胞进行评分，以确定“理论分裂率”。在每张载玻片上观察到的所有双核淋巴细胞中，对含有一个、两个或更多微核的微核化细胞进行评分，以确定“理论微核率”。然后，通过将显微镜载玻片划分为500个淋巴细胞的随机区域，并记录单核、双核细胞，计算“实验分裂率”。通过将显微镜载玻片划分为500个双核细胞的随机区域，并记录含有一个或多个微核的微核化细胞，确定“实验微核率”。通过检查4000个细胞组获得的“实验分裂率”被证明不能代表相应的“理论率”（卡方检验的统计学差异，p<0.05）。通过检查500个双核细胞组获得的“实验微核率”被证明能够代表相应的“理论微核率”（卡方检验无统计学差异，p>0.05）。
