使用启动子探针质粒从栖牙短小杆菌草状亚种中分离强启动子。
Isolation of strong promoters from Clavibacter xyli subsp. cynodontis using a promoter probe plasmid.
作者信息
Haapalainen M, Karp M, Metzler M C
机构信息
Department of Biology, University of Turku, Finland.
出版信息
Biochim Biophys Acta. 1996 Mar 1;1305(3):130-4. doi: 10.1016/0167-4781(95)00203-0.
To isolate promoters from Clavibacter xyli subsp. cynodontis (C. xyli subsp. cynodontis), we constructed a new promoter probe plasmid and made a C. xyli subsp. cynodontis promoter probe library. Two promoters gave over 2500-times stronger expression than the parental plasmid. The promoters were sequenced and compared to other bacterial promoters. These C. xyli subsp. cynodontis promoter regions are GC-rich and do not resemble E. coli promoters, but do resemble a few individual promoters found in streptomycetes.
为了从栖牙短小杆菌嗜犬亚种（栖牙短小杆菌嗜犬亚种）中分离启动子，我们构建了一种新的启动子探针质粒，并制作了栖牙短小杆菌嗜犬亚种启动子探针文库。有两个启动子的表达比亲本质粒强2500倍以上。对这些启动子进行了测序，并与其他细菌启动子进行了比较。这些栖牙短小杆菌嗜犬亚种的启动子区域富含GC，与大肠杆菌启动子不同，但与链霉菌中发现的一些个别启动子相似。
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