栖木短小杆菌嗜犬亚种中核糖体RNA编码基因的鉴定与表征

Identification and characterization of the ribosomal RNA-encoding genes in Clavibacter xyli subsp. cynodontis.

作者信息

Sathyamoorthy M, Alcorn S C, Lohnas G L, Anderson J J, Uratani B B

机构信息

Crop Genetics International, Hanover, MD 21076.

出版信息

Gene. 1991 Dec 1;108(1):47-53. doi: 10.1016/0378-1119(91)90486-u.

DOI:10.1016/0378-1119(91)90486-u

PMID:1761230

Abstract

Clavibacter xyli subsp. cynodontis (Cxc) is a xylem-inhabiting bacterial endophyte of Bermudagrass. This organism is classified with Gram-positive, high G + C content, coryneform-actinomycete bacteria. Southern-blot analysis showed that Cxc contains only one copy of the ribosomal RNA-encoding genes (rRNA). A clone containing the rRNA genes was isolated from a genomic library of Cxc DNA cloned in the lambda EMBL3 vector. The gene cluster was partially sequenced, revealing the gene order 5'-16S-23S-5S-3', similar to that found in other prokaryotes. Low-resolution S1 mapping suggested multiple transcription start points of the rRNA operon.

摘要

栖木糖多孢菌犬齿亚种（Cxc）是百慕大草的一种木质部内生细菌。该生物体被归类为革兰氏阳性、高G + C含量的棒状放线菌。Southern杂交分析表明，Cxc仅含有一份核糖体RNA编码基因（rRNA）。从克隆于λEMBL3载体的Cxc DNA基因组文库中分离出一个含有rRNA基因的克隆。对该基因簇进行了部分测序，揭示了基因顺序为5'-16S-23S-5S-3'，与其他原核生物中的情况相似。低分辨率S1作图表明rRNA操纵子有多个转录起始点。