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本文引用的文献

1
Expression and subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in light-grown Euglena gracilis and three nonchlorophyllous cell lines.四吡咯合成酶胆色素原脱氨酶在光照培养的纤细裸藻及三种无叶绿素细胞系中的表达与亚细胞定位
Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):63-7. doi: 10.1073/pnas.88.1.63.
2
The presequence of Euglena LHCPII, a cytoplasmically synthesized chloroplast protein, contains a functional endoplasmic reticulum-targeting domain.眼虫藻光捕获叶绿素a/b结合蛋白II（一种在细胞质中合成的叶绿体蛋白）的前导序列包含一个功能性内质网靶向结构域。
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11845-9. doi: 10.1073/pnas.90.24.11845.
3
Differences between lumen targeting domains of chloroplast transit peptides determine pathway specificity for thylakoid transport.叶绿体转运肽的内腔靶向结构域之间的差异决定了类囊体转运的途径特异性。
J Biol Chem. 1994 Apr 8;269(14):10189-92.
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Envelope membrane proteins that interact with chloroplastic precursor proteins.与叶绿体前体蛋白相互作用的包膜膜蛋白。
Plant Cell. 1994 Jan;6(1):93-105. doi: 10.1105/tpc.6.1.93.
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Targeting of proteins into and across the thylakoid membrane--a multitude of mechanisms.蛋白质靶向进入类囊体膜及穿过类囊体膜——多种机制
Plant Mol Biol. 1994 Oct;26(1):15-24. doi: 10.1007/BF00039516.
6
The polyprotein precursor to the Euglena light-harvesting chlorophyll a/b-binding protein is transported to the Golgi apparatus prior to chloroplast import and polyprotein processing.眼虫捕光叶绿素a/b结合蛋白的多蛋白前体在叶绿体导入和多蛋白加工之前被转运到高尔基体。
J Biol Chem. 1995 Jun 2;270(22):13084-90. doi: 10.1074/jbc.270.22.13084.
7
Nucleotide sequence of two cDNAs encoding fucoxanthin chlorophyll a/c proteins in the diatom Odontella sinensis.编码中华齿缘藻岩藻黄素叶绿素a/c蛋白的两个cDNA的核苷酸序列。
Plant Mol Biol. 1995 Feb;27(4):825-8. doi: 10.1007/BF00020236.
8
Events surrounding the early development of Euglena chloroplasts. Structure of the developing proplastid in the first hours of illumination from serial sections of wild-type cells.围绕眼虫叶绿体早期发育的事件。从野生型细胞的连续切片观察光照最初几小时内发育中的前质体结构。
J Ultrastruct Res. 1980 Oct;73(1):77-90. doi: 10.1016/0022-5320(80)90117-3.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
10
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.用于分离1至100 kDa范围内蛋白质的三羟甲基氨基甲烷-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳
Anal Biochem. 1987 Nov 1;166(2):368-79. doi: 10.1016/0003-2697(87)90587-2.

一种可溶性蛋白质作为膜结合前体被导入眼虫叶绿体。

A soluble protein is imported into Euglena chloroplasts as a membrane-bound precursor.

作者信息

Sulli C, Schwartzbach S D

机构信息

School of Biological Sciences, University of Nebraska, Lincoln 68588, USA.

出版信息

Plant Cell. 1996 Jan;8(1):43-53. doi: 10.1105/tpc.8.1.43.

DOI:10.1105/tpc.8.1.43
PMID:8597659
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC161080/
Abstract

The Euglena precursor to the small subunit of ribulose-15-bisphosphate carboxylase/oxygenase (pSSU) is a polyprotein. To determine the transport route from cytoplasm to chloroplast, Euglena was pulse labeled with 35S-sulfate and the organelles were separated on sucrose gradients. After a pulse, pSSU was found in the endoplasmic reticulum (ER) and Golgi apparatus. During a chase, ER-and Golgi-localized pSSU decreased concomitant with the appearance of SSU in chloroplasts. SSU was not found in pSSU-containing ER and Golgi fractions. Na2CO3 did not remove pSSU from ER or Golgi membranes, indicating that it was an integral membrane protein. pSSU was inserted in vitro into canine microsomes, and Na2CO3 did not remove pSSU from the microsomal membrane. The in vivo and in vitro experiments show that Euglena pSSU is inserted into the ER membrane and transported as an integral membrane protein to the Golgi apparatus before chloroplast import and polyprotein processing.

摘要

1,5 - 二磷酸核酮糖羧化酶/加氧酶小亚基（pSSU）的眼虫前体是一种多蛋白。为了确定从细胞质到叶绿体的运输途径，用眼虫进行了35S - 硫酸盐脉冲标记，并在蔗糖梯度上分离细胞器。脉冲后，在内质网（ER）和高尔基体中发现了pSSU。追踪过程中发现，ER和高尔基体定位的pSSU减少，同时叶绿体中出现了SSU。在含有pSSU的ER和高尔基体组分中未发现SSU。Na2CO3不能从ER或高尔基体膜上去除pSSU，表示它是一种整合膜蛋白。pSSU在体外插入犬微粒体中时，Na2CO3也不能从微粒体膜上去除pSSU。体内和体外实验表明，眼虫pSSU插入ER膜并作为整合膜蛋白转运到高尔基体，之后再进行叶绿体导入和多蛋白加工。