Kroth-Pancic P G
Institut für Biochemie der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Germany.
Plant Mol Biol. 1995 Feb;27(4):825-8. doi: 10.1007/BF00020236.
Two cDNA clones encoding fucoxanthin chlorophyll a/c-binding proteins (FCP) in the diatom Odontella sinensis have been cloned and sequenced. The derived amino acid sequences of both clones are identical, comparison of the corresponding nucleic acids reveals differences only in the third codon position, suggesting a recent gene duplication. The derived proteins are similar to the chlorophyll a/b-binding proteins of higher plants. The presequences for plastid import resemble signal sequences for cotranslational import rather than transit peptides of higher plants. They are very similar to the presequences of FCP proteins in the diatom Phaeodactylum, but different from the presequences of the gamma-subunit of CF0CF1 of Odontella and the peridinin chlorophyll a binding proteins (PCP) of the dinoflagellate Symbiodinium.
已克隆并测序了中华齿缘藻中两个编码岩藻黄质叶绿素a/c结合蛋白(FCP)的cDNA克隆。两个克隆推导的氨基酸序列相同,相应核酸的比较仅在第三密码子位置发现差异,表明这是一个近期发生的基因重复事件。推导的蛋白质与高等植物的叶绿素a/b结合蛋白相似。质体导入的前导序列类似于共翻译导入的信号序列,而不是高等植物的转运肽。它们与硅藻三角褐指藻中FCP蛋白的前导序列非常相似,但与中华齿缘藻CF0CF1的γ亚基的前导序列以及甲藻共生藻的多甲藻叶绿素a结合蛋白(PCP)的前导序列不同。