Belmont L D, Mitchison T J
Department of Biochemistry, University of California, San Francisco, 94143-0448, USA.
Cell. 1996 Feb 23;84(4):623-31. doi: 10.1016/s0092-8674(00)81037-5.
Using a polymerization inhibition assay, we have purified a small, heat stable protein that physically interacts with tubulin dimers and increases the catastrophe rate of microtubules. Sequence analysis identified this protein as oncoprotein 18 (Op18)/stathmin, a conserved phosphoprotein that is highly expressed in leukemia cells. Immunodepletion experiments in Xenopus egg extracts showed that Op18/stathmin is involved in physiological regulation of mitotic microtubule dynamics. Op18/stathmin is a microtubule regulator that preferentially interacts with unpolymerized subunits. It is a candidate for increasing the microtubule catastrophe rate in mitosis and might also regulate microtubule dynamics in response to external signals.
利用聚合抑制试验，我们纯化了一种小的热稳定蛋白，该蛋白与微管蛋白二聚体发生物理相互作用，并增加微管的解聚速率。序列分析确定该蛋白为癌蛋白18（Op18）/ 微管相关蛋白，这是一种在白血病细胞中高度表达的保守磷蛋白。非洲爪蟾卵提取物中的免疫去除实验表明，Op18 / 微管相关蛋白参与有丝分裂微管动力学的生理调节。Op18 / 微管相关蛋白是一种微管调节剂，优先与未聚合的亚基相互作用。它是有丝分裂中增加微管解聚速率的一个候选蛋白，也可能响应外部信号调节微管动力学。
