Suzuki K, Nagao K, Tokunaga J, Hirosawa M, Tsubone H, Uyeda M
Laboratory of Medicinal Microbiology, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
J Enzyme Inhib. 1995;9(4):243-52. doi: 10.3109/14756369509036553.
A new inhibitor of DNA methyltransferase named DMI-1 has been discovered in the culture filtrate of Streptomyces sp. strain No. 560. DMI-1 was purified by extraction with ethyl acetate followed by Diaion HP-20SS and silica gel column chromatography. The structure of DMI-1 was determined to be 8-methylpentadecanoic acid (C16H32O2). DMI-1 is a novel inhibitor of methyltransferase isolated from microorganisms and is structurally different from sinefungin and A9145C which are structural analogs of S-adenosylmethionine (methyl donor). DMI-1 was a strong inhibitor of N6-methyladenine-DNA methyltransferase (M. Eco RI, EC 2.1.1.72) in a noncompetitive manner and its inhibition depended on the pH and temperature in the assay media.
在链霉菌属560号菌株的培养滤液中发现了一种名为DMI-1的新型DNA甲基转移酶抑制剂。通过乙酸乙酯萃取，随后经Diaion HP-20SS和硅胶柱色谱法对DMI-1进行纯化。DMI-1的结构被确定为8-甲基十五烷酸（C16H32O2）。DMI-1是一种从微生物中分离得到的新型甲基转移酶抑制剂，在结构上与作为S-腺苷甲硫氨酸（甲基供体）结构类似物的杀稻瘟菌素和A9145C不同。DMI-1以非竞争性方式强烈抑制N6-甲基腺嘌呤-DNA甲基转移酶（M. Eco RI，EC 2.1.1.72），其抑制作用取决于测定介质中的pH值和温度。
