放线菌产生的新型丝氨酸羧肽酶抑制剂贝拉汀A和B。I. 分类学、生产、分离及生物学活性
Belactins A and B, new serine carboxypeptidase inhibitors produced by Actinomycete. I. Taxonomy, production, isolation and biological activities.
作者信息
Murakami S, Harada S, Kojima F, Kinoshita N, Takahashi Y, Hamada M, Takeuchi T, Aoyagi T
机构信息
Institute of Microbial Chemistry, Tokyo, Japan.
出版信息
J Enzyme Inhib. 1995;9(4):263-75. doi: 10.3109/14756369509036555.
Belactins A and B, new inhibitors of serine carboxypeptidase were discovered in the fermentation broth of Saccharopolyspora sp. MK19-42F6. They were purified by ethyl acetate extraction, silica gel chromatography, Sephadex LH20 chromatography, Capcellpak C18 SG120 reversed phase HPLC and centrifugal partition chromatography (CPC) following their inhibitory activity against carboxypeptidase Y (CP-Y). The inhibition constants (Ki) of belactins A and B against CP-Y are 0.14 and 0.27 microM respectively. Belactins A and B have highly specific inhibitory activities for CP-Y among various peptidases, have no antimicrobial activities at 100 micrograms/ml and have low toxicities.
在糖多孢菌属菌株MK19 - 42F6的发酵液中发现了丝氨酸羧肽酶的新型抑制剂贝拉汀A和B。根据它们对羧肽酶Y（CP - Y）的抑制活性，通过乙酸乙酯萃取、硅胶柱色谱、葡聚糖凝胶LH20柱色谱、Capcellpak C18 SG120反相高效液相色谱和离心分配色谱（CPC）对其进行纯化。贝拉汀A和B对CP - Y的抑制常数（Ki）分别为0.14和0.27微摩尔。在各种肽酶中，贝拉汀A和B对CP - Y具有高度特异性抑制活性，在100微克/毫升时无抗菌活性且毒性较低。
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