Kau N, Günther M, Fahnenstich H, Kowalewski S, Bartmann P
Department of Neonatology, University of Bonn, Germany.
Acta Anaesthesiol Scand Suppl. 1995;107:107-11. doi: 10.1111/j.1399-6576.1995.tb04343.x.
The alternative use of flame photometry and ion selective electrodes for electrolyte determinations in neonatology led to the question how the two methods agree. 104 routine blood samples were taken as matched pairs for a comparison of sodium and potassium determinations. Pre-analytical error was avoided by the mode of blood collection. The influence of the protein and lipid content of blood on the differences between the methods was determined by calculating its water content and making a post-correction of the flame values. Thus the differences for sodium could be reduced, whereas the differences for potassium slightly increased. As long as the imprecision of sodium determination is a meaningful methodological problem, the influence of the protein and lipid content of neonatal blood is not the main factor for differences between ISE and flame photometry.
在新生儿科中，火焰光度法和离子选择性电极法交替用于电解质测定，这引发了两种方法一致性如何的问题。采集了104对常规血样用于比较钠和钾的测定。通过采血方式避免了分析前误差。通过计算血液的含水量并对火焰值进行后校正，确定了血液中蛋白质和脂质含量对两种方法差异的影响。这样一来，钠的差异可以减小，而钾的差异略有增加。只要钠测定的不精密度是一个有意义的方法学问题，新生儿血液中蛋白质和脂质含量的影响就不是离子选择性电极法和火焰光度法之间差异的主要因素。
